Napoli have not included new recruit Victor Osimhen and Algerian defender Faouzi Ghoulam in their UEFA Champion league 2020/21 squad.
The Serie A giants will face Barcelona in the round of 16 of the tournament in a one-legged tie on August 8.
According to the list announced by Uefa, Senegal international Koulibaly is the only African star who made the club’s 23-man squad.
Koulibaly has featured 33 times for Napoli across all competitions, including six in the European tournament and will hope to help his side win the trophy.
Another dropped African player is Algeria international, Ghoulam, who will play no further part in the competition as he is still recovering from an injury.